Sample Preparation (9)
- How many proteins can be identified from a single gel band?
- Can I submit a silver-stained gel?
- Which stains (other than Coomassie blue) are compatible with mass spectrometry?
- Do you use only trypsin to digest proteins or can other proteolytic enzymes be used?
- I have detergent in my sample. Are there ways to remove it?
- I have my protein complex in a buffer with very high salt concentration plus 5% glycerol. Is this buffer compatible with mass spectrometry?
- Can I use in-house Coomassie blue stain? Is there a preferred protocol ?
- Are there any good papers describing phosphoproteomics?
- How do I submit a sample to be run?